Anti-CD80 [16C10]

• Catalog Number: orb758984
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD80
• Target: CD80
• Clonality: Monoclonal
• Species/Host: Monkey
• Isotype: Monkey IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.
• UniProt ID: P33681
• Tested applications: Blocking, ELISA
• Clone Number: 16C10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B7.1; CD28L; CD28LG; B7-1; B7; BB1; B7/BB1;T-lymph
• Note: For research use only

Flow cytometry using the anti-CD80 antibody 16C10. Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of 16C10 (orb758984, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody 16C10. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 16C10 (orb758984) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758984, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.