Anti-CD45RA or A/B [OX-30]

SKU: orb2645689

Description

Anti-CD45RA or A/B [OX-30]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, FC, IHC-Fr
• Reactivity: Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: OX-30
• Immunogen: This antibody was prepared by immunizing BALB/c mice with rat lymph node glycoproteins.
• Target: CD45RA or A/B
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PTPRC; B220; CD45; CD45 R; GP180; L-CA; LCA; LY5; T200; protein tyrosine phosphatase; receptor type C; Receptor-type tyrosine-protein phosphatase C

Flow-cytometry using anti-CD45RA or A/B (Receptor-type tyrosine-protein phosphatase C isoform) antibody OX-30 Staining of rat (Rattus norvegicus) lymphocytes with either an isotype control (A) or the rabbit-chimeric version of OX-30 (B, orb2645688) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed rat splenocytes with anti-CD45RA or A/B (Receptor-type tyrosine-protein phosphatase C) antibody OX-30. Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of OX-30 (orb2645688) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645688, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD45RA or A/B (Receptor-type tyrosine-protein phosphatase C) antibody OX-30 Rat thymus lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of OX-30 (orb2645688) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for unmodified CD45RA is ~143.3kDa, but this protein is also highly glycosylated (UniProt Id: P04157). orb2645688 successfully detected CD45RA or A/B in rat thymus tissue lysate.

Quick Database Links

Gene Symbol

CD45RA or A/B

UniProt

P04157