Anti-CD24 [M1/69]

SKU: orb2645584

Description

Anti-CD24 [M1/69]

Research Area

Cancer Biology, Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	FC, IF, IHC, WB
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG
Clone No.	M1/69
Immunogen	The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.
Target	CD24
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen

Quality Guarantee

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Immunofluorescence staining of RAW264.7 cells with anti-CD24 antibody M1/69. Immunofluorescence analysis of paraformaldehyde-fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of M1/69 (orb2645584) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645584, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-CD24 antibody M1/69. Mouse spleen tissue lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the chimeric rabbit version of M1/69 (orb2645584) at 0.0003 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.