Anti-CD19 [MB19-1]

• Catalog Number: orb1821631
• Category: Antibodies
• Description: Anti-CD19 [MB19-1]
• Target: CD19
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Canine, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.
• UniProt ID: P25918
• Tested applications: ELISA, FC, IF
• Clone Number: MB19-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B-lymphocyte antigen CD19; Differentiation antigen
• Note: For research use only

Flow cytometry using the Anti-CD19 antibody MB19-1. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of MB19-1 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-CD19 MB19-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MB19-1 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue).