Anti-Beta Dystroglycan [P38G1]

• Catalog Number: orb1821529
• Category: Antibodies
• Description: Anti-Beta Dystroglycan [P38G1]
• Target: Beta Dystroglycan
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a mixture of transfected HEK 293 cells expressing human and mouse β-DAG.
• UniProt ID: Q62165, Q14118
• Tested applications: ELISA, FC
• Clone Number: P38G1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Dystroglycan 1; Dag1; Beta-DG; β-DAG; BETA DAG; Dy
• Note: For research use only

Flow cytometry using anti-Beta Dystroglycan antibody P38G1. MCF7 cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of P38G1 (orb1821529, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of A431 cells with anti-Beta Dystroglycan antibody P38G1. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of P38G1 (orb1821529) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell membrane/junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821529, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Beta Dystroglycan antibody P38G1. Human skeletal muscle tissue lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of P38G1 (orb1821529) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.