Aconitase 1 (pS138) Antibody

SKU: orb2649826

Description

Rabbit polyclonal antibody to Aconitase 1 (pS138)

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: ACO1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

Western blot analysis of Aconitase 1 (pS138) expression in Hela (A) whole cell lysates.

Immunohistochemical analysis of Aconitase 1 (pS138) staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Aconitase 1 (pS138) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P21399