ANK1 Antibody

SKU: orb1246659

Description

ANK1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA
• Predicted Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QIVKRASLKRGKQ
• Target: ANK1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R

Quick Database Links

Gene Symbol

ANK1

UniProt

P16157

RefSeq (Protein):NP_065209.2, NP_000028.3, NP_065213.2, NP_065210.2, NP_065211.2, NP_065208.2