Alpha-B Crystallin ELISA Kit

SKU: orb1822519

Description

Colorimetric detection of alpha B Crystallin

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Application Notes:
  1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

Key Properties

• Reactivity: Human
• Sample Types: Cell Lysates, Tissue, Serum
• Assay Type: Sandwich
• Range: 0.352 - 22.5 ng/ml
• Sensitivity: 0.009 ng/ml

Storage & Handling

• Storage: 4°C and -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AACRYA, CRYA2, CRYAB, CTPP2, HSPB5, NY Ren 27 antigen, alpha crystallin B

Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.352 - 22.5 ng/mL.

Quick Database Links

Gene Symbol

Alpha-B Crystallin ELISA Kit