A2M Antibody

SKU: orb750396

Description

alpha 2 Macroglobulin antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Alpha-2-Macroglobulin has been tested by western blot and is suitable to be assayed against 1.0 ug of a2-Macroglobulin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:32,000 to 1:160,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: a2-Macroglobulin [Human Plasma]
• Target: A2M
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 82 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Alpha-2-Macroglobulin Antibody, goat anti-A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody, Alpha-2-M, PZP-like alpha-2-macroglobulin domain-containing protein 5

Biorbyt Goat anti Alpha-2-Macroglobulin was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 649 conjugated Donkey anti goat (1:10K in TBS/orb348637) 1 hr RT.

Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 2-8°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.

Quick Database Links

Gene Symbol

A2M

UniProt

P01023

RefSeq (Protein):NP_000005.2