E-64d

SKU: orb1301127

Description

Aloxistatin (E64d) is a cell-permeable, irreversible inhibitor of cysteine proteases, including calpain, and inhibits platelet aggregation. It is widely used in research to study protease function in cellular models and in vivo, particularly in neuroscience and cardiovascular studies.

Research Area

Cell Biology

Key Properties

• CAS Number: 88321-09-9
• MW: 342.43
• Purity: >98%
• Formula: C₁₇H₃₀N₂O₅
• SMILES: CCOC(=O)[C@H]1O[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)NCCC(C)C
• Target: Protease
• Solubility: Soluble in DMSO (up to 20 mg/ml) or in Ethanol (up to 10 mg/ml)

Bioactivity

• In Vivo:
  Aloxistatin (100 mg/kg, p.o.) strongly inhibits the cathepsin B&L activities in the skeletal muscle, heart and liver of hamsters. In spinal cord injury (SCI) rats, Aloxistatin provides neuroprotection in SCI lesion and penumbra. Aloxistatin reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B activity.
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• In Vitro:
  Aloxistatin can enter the intact platelet and inhibit proteolysis by inhibiting calpain. Aloxistatin blunts Parathyroid hormone (PTH)-induced cell proliferation and inhibits differentiation osteoblasts in vitro.
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• Cell Research:
  Aloxistatin (E64d) is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.1 %) before use. Cell proliferation and apoptosis are assessed by staining for a proliferation marker, Ki67, or an apoptotic marker, cleaved caspase 3, following the protocol described above for the polarity markers. MCF10 variants are grown in 3D rBM overlay cultures for 4 days and are treated with 0.1 % DMSO, 5 μM CA074Me or 5 μM Aloxistatin. The percentage of structures that are positive for Ki67 or cleaved caspase 3 is determined by counting a total of 100 structures on two separate coverslips with a Zeiss Axiophot epifluorescent microscope. Structures are considered Ki67 positive if they contained at least one cell staining for Ki67. Structures are considered to be caspase 3 positive if they contained at least one cell that is positive for cleaved caspase 3 and the positive cell(s) is not localized in the center of a developing lumen.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Aloxistatin; Loxistatin

Compound Identifiers

PubChem CID

65663