AkaLumine hydrochloride

SKU: orb1706391

Description

AkaLumine hydrochloride is a fluorescein derivative that binds recombinant fluorescent protein with a Km of 2.06 µM. It serves as a versatile fluorescent probe for in vitro assays and in vivo imaging applications across molecular and cellular biology research.

Key Properties

• CAS Number: 2558205-28-8
• MW: 338.85
• Purity: 99.32% (May vary between batches)
• Formula: C₁₆H₁₉ClN₂O₂S
• SMILES: Cl.CN(C)c1ccc(\C=C\C=C\C2=N[C@H](CS2)C(O)=O)cc1
• Target: Others
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:5 mg/mL (14.76 mM);H2O:25 mg/mL (73.78 mM);DMSO:120 mg/mL (354.14 mM)

Bioactivity

• Target IC50:
  Recombinant Fluc protein:2.06 μM
• In Vivo:
  AkaLumine hydrochloride-treated mice 15 min after intravenous injection of LLC/luc cells at the dose of 5 mM, exhibit a 3.3-fold increase in detection sensitivity of disseminated cancer cells in the lung compared with the experimental control group. In the inverse order at an 8 h interval. AkaLumine hydrochloride displays about a fourfold increase in signals from lung metastasis compared with the experimental control group.
• In Vitro:
  In both LLC/luc and MDA-MB-231/Luc cells exposed to AkaLumine hydrochloride, the signal peaks at lower concentrations (starting from 2.5 μM). Conversely, the bioluminescence produced by D-luciferin and CycLuc1 continuously increases with concentration, without reaching saturation, even at 250 μM.
• Animal Research:
  Instructions I. Solution preparation 1. Stock solution preparation Dissolve AkaLumine hydrochloride in deionized water or dimethyl sulfoxide (DMSO). 2. Working solution preparation: Prepare a stock solution of no more than 50 mg/mL, with a common concentration of 40 mg/mL. For example, 40 mg AkaLumine hydrochloride can be dissolved in 1 mL deionized water or DMSO. Notes: Storage: It is recommended to prepare and use immediately; if storage is required, it should be stored at low temperature to ensure stability. II. In vivo imaging: 1. Administration: Inject the prepared AkaLumine hydrochloride solution into experimental animals by intraperitoneal injection. 2. Dosage: The specific dose is adjusted according to the experimental situation, generally ranging from 50–200 mg/kg. 3. Imaging: After injection, use an imaging system that can detect NIR luminescence for bioluminescence imaging.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dye reagent, AkaLumine hydrochloride, AkaLumine Hydrochloride, AkaLumine hydrochloride (1176235-08-7 free base), 1176235-08-7, 1176235-08-7 free base, recombinant Fluc protein

Compound Identifiers

PubChem CID

124203055