AI-10-49

SKU: orb1300497

Description

AI-10-49 is a potent small molecule that selectively disrupts the interaction between the CBFβ-SMMHC oncoprotein and RUNX1, exhibiting an IC50 of 260 nM. This inhibitor is a valuable research tool for investigating acute myeloid leukemia (AML) pathogenesis and evaluating therapeutic strategies in cellular models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 1256094-72-0
• MW: 660.52
• Purity: 98.75%
• Formula: C₃₀H₂₂F₆N₆O₅
• SMILES: FC(F)(F)Oc1ccc2nc([nH]c2c1)-c1ccc(OCCOCCOc2ccc(nc2)-c2nc3ccc(OC(F)(F)F)cc3[nH]2)cn1
• Target: Others,DNA/RNA Synthesis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5 mM);DMSO:93 mg/mL (140.8 mM);Ethanol:93 mg/mL (140.8 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  CBFβ-SMMHC:260 nM
• In Vivo:
  In mice transplanted with Cbfb+/MYH11;Nras+/G12D leukemic cells, AI-10-49 (200 mg/kg, i.p.) reduces leukemia expansion.
• In Vitro:
  AI-10-49 displays specific growth inhibition of inv(16)-positive cell line ME-1. AI-10-49 selectively binds to CBFβ-SMMHC, disrupts its binding to RUNX1, and restores RUNX1 transcriptional activity.
• Cell Research:
  Cell lines are cultured in IMDM supplemented with 10%~20% fetal bovine serum (FBS) according to culture conditions indicated by ATCC and 1% penicillin/streptomycin (Pen/Strep). All cell lines are tested for mycoplasma. Cells are cultured at 300,000 cells per ml in 96 well plates for 24 and 48 hours in DMSO, or different of AI-10-47, AI-410-49, AI-4-57, or AI-4-88); each in duplicate or triplicate. Cell viability is evaluated using DAPI by flow cytometry. Data is analyzed using FlowJo software and Graphpad Prism software.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, AI1049, AI-10-49, AI 10 49, DNASynthesis, DNA Synthesis, CBFβ-SMMHC, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

49806644