ADH1B Antibody

• Catalog Number: orb1263684
• Category: Antibodies
• Description: ADH1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-237 amino acids from the Central region of human ADH1B.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: ADH1B
• UniProt ID: P00325
• NCBI: P00325
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Alcohol dehydrogenase 1B, Alcohol dehydrogenase su
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of ADH1B Antibody in HepG2 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ADH1B Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).