Acid Yellow 9 monosodium salt

SKU: orb1307233

Description

Acid Yellow 9 monosodium salt is an azo dye utilized as a model pollutant in environmental microbiology research. It serves as a sole source of nitrogen, carbon, and energy for Pseudomonas fluorescens, making it valuable for studying microbial degradation pathways in both in vitro and bioremediation applications.

Key Properties

• CAS Number: 74543-21-8
• MW: 379.34
• Purity: 99.64% (May vary between batches)
• Formula: C₁₂H₁₀N₃NaO₆S₂
• SMILES: [Na+].Nc1ccc(cc1S(=O)(=O)O)\N=N\c2ccc(cc2)S(=O)(=O)[O-]
• Target: Others
• Solubility: DMSO:55 mg/mL (144.99 mM)

Bioactivity

• In Vitro:
  I. For bacterial degradation studies 1. Cultivate bacteria: Cultivate Pseudomonas fluorescens in a suitable nutrient medium. 2. Add dye: Add Acid Yellow 9 monosodium salt (usually at a concentration of 50-200 mg/L) to the medium to provide the dye as the only source of carbon and nitrogen. 3. Cultivate: Cultivate at a suitable growth temperature (usually 30-37°C) and monitor bacterial growth and dye degradation. 4. Monitor degradation: Use an ultraviolet-visible spectrophotometer (UV-Vis) or liquid chromatography (HPLC) to detect the reduction in dye concentration or degradation products. II. For bioremediation studies 1. Contaminated water sample: Prepare wastewater or water sample containing Acid Yellow 9 with a known dye concentration. 2. Inoculate bacteria: Inoculate Pseudomonas fluorescens or other suitable microorganisms into the wastewater. 3. Monitor degradation: Regularly monitor the degradation of Acid Yellow 9 and track changes in dye concentration using a spectrophotometer or chromatography. 4. Evaluation of remediation effect: Evaluate the effect of microbial treatment by analyzing the degradation of dyes and comparing with control samples. III. For environmental toxicity testing: 1. Toxicity test: Expose different concentrations of Acid Yellow 9 monosodium salt to Pseudomonas fluorescens or other environmental microorganisms to monitor bacterial growth and metabolic activity. 2. Biodegradation evaluation: Evaluate the degradation ability and growth of microorganisms in the presence of the dye by tracking changes in dye concentration. IV. For dye and pigment research 1. Dye exposure study: Expose different concentrations of Acid Yellow 9 monosodium salt to microbial culture medium to study the degradation pathway of microorganisms. 2. Enzyme activity analysis: Use enzyme activity assay to evaluate the enzyme system of bacteria that decompose dyes and further explore its degradation mechanism.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acid Yellow 9, Acid Yellow 9 monosodium, Acid Yellow 9 monosodium salt, inhibit, Hydrogen 4-aminoazobenzene-3,4'-disulphonate, Hydrogen 4-aminoazobenzene-3,4'-disulphonate (sodium salt), Inhibitor

Compound Identifiers

PubChem CID

44152902