ACADS Antibody

• Catalog Number: orb1256516
• Category: Antibodies
• Description: ACADS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 44kDa
• Target: ACADS
• UniProt ID: P16219
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ACADS, ACAD3, SCAD
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ACADS antibody (orb1256516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunofluorescence analysis of MCF7 cells using ACADS antibody (orb1256516). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug ACADS antibody (orb1256516). Western blot was performed from the immunoprecipitate using ACADS antibody (orb1256516) at a dilution of 1:1000.