ABCB4/MDR3 Antibody

SKU: orb1537134

Description

ABCB4/MDR3 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P
• Dilution Range: ELISA (1:100 - 1:200), IHC (1:100 - 1:200), IHC-P (1:50)
• Reactivity: Human
• Application Notes:
  Further information: Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Dilute antibody at 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant Human MDR3
• Target: ABCB4 / MDR3
• Purification: Protein A/G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris, pH 7.3-7.7, 1% rAlbumin, < 0.1% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ABCB4, ABC21, ICP3, GBD1, MDR3, MDR2, Multidrug resistance protein 3, Multiple drug resistance 3, P-glycoprotein 3, Pgy2, PGY3, PFIC-3, MDR2/3

Quick Database Links

Gene Symbol

ABCB4 / MDR3