9-Methoxycamptothecin

SKU: orb1301268

Description

9-Methoxycamptothecin (MCPT) is a topoisomerase I inhibitor with demonstrated antitumor properties. This camptothecin derivative is widely used in oncology research for investigating cytotoxic mechanisms and evaluating efficacy in both in vitro cell-based assays and in vivo animal models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 39026-92-1
• MW: 378.38
• Purity: 98.49%
• Formula: C₂₁H₁₈N₂O₅
• SMILES: CC[C@@]1(O)C(=O)OCc2c1cc1-c3nc4cccc(OC)c4cc3Cn1c2=O
• Target: Apoptosis,Topoisomerase
• Solubility: 10% DMSO+90% Corn Oil:1 mg/mL (2.64 mM);DMSO:50 mg/mL (132.14 mM)

Bioactivity

• In Vitro:
  9-Methoxycamptothecin(MCPT) induced cytotoxicity in seven human cancer cell lines in a dose dependent manner after 72h, with A2780 and Hela cell lines more sensitive, so the two cell lines were chosen to do further studies. MCPT induced strong G2/M arrest in both A2780 cells and Hela cells after 24h, following by substantial sub-G1 arrest (indicating apoptosis). The apoptosis was verified by staining with Annexin V-FITC and propidium iodide. ROS generation increased significantly in MCPT-induced apoptosis. Meanwhile, the apoptosis appeared to be dependent on caspase-3, -8 and -9 in A2780 cells, and caspase-3 in Hela cells. In addition, MCPT induced up-regulation expression of most of seventeen genes in both cell lines. Western blot verified that changes of TNFα, Fas, P53 and P27 protein level were consistent with their gene expression changes.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, Apoptosis, 9 Methoxycamptothecin, 9Methoxycamptothecin, 9-Methoxycamptothecin, Topoisomerase

Compound Identifiers

PubChem CID

123617