6-Thio-2'-Deoxyguanosine

SKU: orb1300504

Description

6-Thio-2'-Deoxyguanosine (β-TGdR) is a nucleoside analog recognized as a substrate for telomerase. It is utilized in research to study telomere biology and telomerase activity in both cellular and biochemical assays, with applications in investigating aging and oncology-related pathways.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 789-61-7
• MW: 283.31
• Purity: 99.44% (May vary between batches)
• Formula: C₁₀H₁₃N₅O₃S
• SMILES: Nc1nc(=S)c2ncn([C@H]3C[C@H](O)[C@@H](CO)O3)c2[nH]1
• Target: DNA/RNA Synthesis
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:1 mg/mL (3.53 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:52 mg/mL (183.54 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  In mice bearing A549 lung cancer xenografts, 6-Thio-dG (2 mg/kg, i.p.) decreases the tumorigenicity of A549 Cells by inducing telomere dysfunction.
• In Vitro:
  6-Thio-dG induces telomere dysfunction and results in progressive telomere shortening in telomerase-positive human cancer cells and hTERT-expressing human fibroblasts. In tested cancer cells, 6-thio-dG inhibits cell viability with IC50 values between 0.7 and 2.9 μM.
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• Cell Research:
  Cells are plated in growth media in 96-well plates. Cells are incubated for 1 week and treated with varying concentrations of 6-thio-dG and 6-thioguanine or DMSO every 3 days. The 96-well plates are analyzed according the manufacturer's directions for the CellTiterGlo luminescent cell viability assay to obtain IC50 values. The IC50 is defined as the concentration of drug at which 50% of the cells are inhibited by the drug. Sigmoidal dose–response curves are used to calculate IC50 values. All samples are analyzed in triplicate, and SDs are from two independent experiments.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RNASynthesis, RNA Synthesis, β-TGdR, DNASynthesis, DNA Synthesis, DNA/RNA Synthesis, 6 Thio 2' Deoxyguanosine, 6-Thio-2'-Deoxyguanosine, 6Thio2'Deoxyguanosine, 6-Thio-dG, b-TGdR, beta-TGdR, inhibit, Inhibitor

Compound Identifiers

PubChem CID

3000603