5-Ethynyluridine

SKU: orb1303878

Description

5-Ethynyluridine is a nucleoside analog used to label newly synthesized RNA for capture via click chemistry (RICK). This method enables the systematic identification of proteins bound to diverse RNA types, including nascent and nonpolyadenylated transcripts, in both in vivo and in vitro experimental systems for transcriptome-wide interaction studies.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 69075-42-9
• MW: 268.22
• Purity: 99.77% (May vary between batches)
• Formula: C₁₁H₁₂N₂O₆
• SMILES: OC[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cc(C#C)c(=O)[nH]c1=O
• Target: Nucleoside Antimetabolite/Analog
• Solubility: DMSO:55 mg/mL (205.06 mM);DMF:10 mg/mL (37.28 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:1 mg/mL (3.73 mM);PBS (pH 7.2):5 mg/mL (18.64 mM)

Bioactivity

• In Vivo:
  EU labelling can be used to examine transcription in vivo in mouse models of nervous system disorders. Injection of EU directly into the cerebellum results in reproducible labelling of newly transcribed RNA in cerebellar neurons and glia, with cell type-specific differences in relative labelling intensities, such as Purkinje cells exhibiting the highest levels. EU-labelling accumulating into cytoplasmic inclusions, indicating that EU, like other modified uridines, may introduce non-physiological properties in labelled RNAs.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Analog, 5Ethynyluridine, 5-Ethynyluridine, 5-EU, 5 Ethynyluridine, neurons, nascent RNA, NucleosideAntimetabolite, Nucleoside Antimetabolite/Analog, Nucleoside Antimetabolite, inhibit, Inhibitor, RNA transcripts, purkinje cells

Compound Identifiers

PubChem CID

14885960