4-MUNANA

SKU: orb1692292

Description

4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid (sodium salt)

Key Properties

• CAS Number: 76204-02-9
• MW: 489.41
• Purity: 99.58%
• Formula: C₂₁H₂₄NNaO₁₁
• SMILES: [Na+].CC(=O)N[C@@H]1[C@@H](O)C[C@](Oc2ccc3c(C)cc(=O)oc3c2)(O[C@H]1[C@H](O)[C@H](O)CO)C([O-])=O
• Target: Others
• Solubility: H2O:80 mg/mL (163.46 mM);DMF:15 mg/mL (30.65 mM);Ethanol:0.25 mg/mL (0.51 mM);PBS (pH 7.2):10 mg/mL (20.43 mM);DMSO:255 mg/mL (521.04 mM)

Bioactivity

• In Vitro:
  Instructions: I. Solution preparation 1. Stock solution: Dissolve 4-MUNANA in a suitable buffer to prepare a stock solution with a concentration of 1–10 mM. The specific concentration can be adjusted according to the experimental requirements. 2. Working solution: Dilute the 4-MUNANA stock solution to a working concentration (usually 100–500 µM). The specific concentration can be adjusted according to the experimental requirements. Notes: Powder: Store at -20°C or lower in a dark, dry environment. Stock solution: Store at -20°C in a dark place after aliquoting. Avoid repeated freezing and thawing. Store at 4°C for a short period of time. II. Operation steps Neuraminidase activity detection 1. Detection buffer: Prepare a buffer suitable for neuraminidase activity, such as 50 mM sodium acetate (pH 5.5). 2. Enzyme reaction: Mix a sample containing neuraminidase (such as a virus sample or cell lysate) with the 4-MUNANA working solution. 3. Incubate at 37°C for 30–60 Minutes (time can be optimized according to the experiment). 4. After the reaction is completed, add an equal volume of stop solution (such as 0.1 M glycine buffer, pH 10.4) to enhance the fluorescence signal. 5. Fluorescence detection Detection method: Use a fluorescence spectrophotometer or microplate reader to detect the fluorescence signal generated by the reaction. Excitation wavelength: ~365 nm Emission wavelength: ~450 nm Notes Standard curve: Use known concentrations of 4-methylumbelliferone to prepare a standard curve for quantitative analysis of neuraminidase activity. Control experiment: Set up negative control (no enzyme) and positive control (containing known inhibitors) to verify the specificity of the detection.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4 Methylumbelliferyl N acetyl α D Neuraminic Acid (sodium salt), 4-Methylumbelliferyl-N-acetyl-a-D-Neuraminic Acid, 4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid, 4MethylumbelliferylNacetylαDNeuraminic Acid (sodium salt), 4-Methylumbelliferyl-N-acetyl-α-D-Neuraminic Acid sodium salt

Compound Identifiers

PubChem CID

23683295