4-Methylumbelliferyl oleate

SKU: orb2900421

Description

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).

Key Properties

• CAS Number: 18323-58-5
• MW: 440.62
• Purity: >98% (HPLC)
• Formula: C₂₈H₄₀O₄
• SMILES: CCCCCCCC\C=C/CCCCCCCC(=O)Oc1ccc2c(C)cc(=O)oc2c1
• Solubility: In Vitro: DMSO : 100 mg/mL (226.96 mM; Ultrasonic )

Bioactivity

• In Vitro:
  The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 μL 0.1 mM 4-MU oleate, 20 μL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 μL of sample solution. Porcine pancreatic lipase (25 μL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37°C for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only