WNT3A

SKU: orb333957

Description

Mouse monoclonal antibody to WNT3A

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 1E6G4
• Immunogen: Purified recombinant fragment of human WNT3A (AA: 170-352) expressed in E. Coli.
• Molecular Weight: 39.4kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Concentration: 1mg/ml
• Disclaimer: For research use only

Immunofluorescence analysis of HeLa cells using WNT3A antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using WNT3A antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded endometrial cancer tissues using WNT3A antibody

Western blot analysis of human recombinant protein using WNT3A antibody

Western blot analysis of HEK293 using WNT3A antibody

Western blot analysis of A549 (Lane1), HepG2 (Lane2), and A431 (Lane3) cell lysate using WNT3A antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using WNT3A antibody

Quick Database Links

NCBI Gene ID

89780