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Catalog Number | orb333957 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to WNT3A |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | Mouse IgG2b |
Conjugation | Unconjugated |
Reactivity | Human |
Concentration | 1mg/ml |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Immunogen | Purified recombinant fragment of human WNT3A (AA: 170-352) expressed in E. Coli. |
MW | 39.4kDa |
Tested applications | ELISA, FC, ICC, IHC, WB |
Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000 |
Clone Number | 1E6G4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Entrez | 89780 |
Immunofluorescence analysis of HeLa cells using WNT3A antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using WNT3A antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded endometrial cancer tissues using WNT3A antibody
Western blot analysis of human recombinant protein using WNT3A antibody
Western blot analysis of HEK293 using WNT3A antibody
Western blot analysis of A549 (Lane1), HepG2 (Lane2), and A431 (Lane3) cell lysate using WNT3A antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of Hela cells using WNT3A antibody
WB | |
Bovine, Canine, Human, Mouse, Porcine, Rabbit, Sheep | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
Greater than 90% as determined by SDS-PAGE. | |
41.3 kDa | |
E.coli |