VE-Cadherin rabbit pAb Antibody

SKU: orb767216

Description

Rabbit polyclonal antibody to Cadherin 5

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CDH5. AA range:391-440
• Molecular Weight: 86
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDH5 antibody, anti-Cadherin-5 antibody, anti-7B4 antigen antibody, anti-Vascular endothelial cadherin antibody, anti-VE-cadherin antibody, anti-CD144 antibody

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western blot analysis of lysates from 1) Hela, 2) mouse-lung , 3) mouse-kidney cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunofluorescence analysis of human-lung tissue. 1, VE-Cadherin Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of human-lung tissue. 1, VE-Cadherin Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.