VE-Cadherin rabbit pAb Antibody

SKU: orb767286

Description

Rabbit polyclonal antibody to Cadherin 5

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDH5. AA range:697-746
• Molecular Weight: 120
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDH5 antibody, anti-Cadherin-5 antibody, anti-7B4 antigen antibody, anti-Vascular endothelial cadherin antibody, anti-VE-cadherin antibody, anti-CD antigen CD144 antibody

Western Blot analysis of Jurkat cells using VE-Cadherin Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000.

Western Blot analysis of A549 using VE-Cadherin Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000.

Western blot analysis of lysates from Jurkat cells, using VE-Cadherin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).