STAT4 Antibody

• Catalog Number: orb1256676
• Category: Antibodies
• Description: STAT4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 76kDa
• Target: STAT4
• UniProt ID: Q14765
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: STAT4, SLEB11
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (orb1256676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectum using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human rectal cancer using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using STAT4 antibody (orb1256676) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using STAT4 antibody (orb1256676). Blue: DAPI for nuclear staining.