SRGN Antibody

SKU: orb1256702

Description

SRGN Antibody

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-158 of human SRGN (NP_002718.2).
• Target: SRGN
• Molecular Weight: Observed: 17kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Serglycin, Hematopoietic proteoglycan core protein, Platelet proteoglycan core protein, PPG, Secretory granule proteoglycan core protein, SRGN, PRG, PRG1

Western blot analysis of extracts of THP-1 cells, using SRGN antibody (orb1256702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using SRGN antibody (orb1256702) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using SRGN antibody (orb1256702). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

SRGN

UniProt

P10124