Recombinant Human LIF

SKU: orb1921830

Description

Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. Initially identified as a factor that inhibits the proliferation of murine myeloid leukemia cells, LIF displays a wide variety of important functions in a cell-, tissue- and context-dependent manner in many physiological and pathological processes, including regulating cell proliferation, pluripotent stem cell self-renewal, tissue/organ development and regeneration, neurogenesis and neural regeneration, maternal reproduction, inflammation, infection, immune response, and metabolism. Emerging evidence has shown that LIF plays an important but complex role in human cancers. While LIF displays a tumor suppressive function in some types of cancers, including leukemia. LIF is overexpressed and exerts an oncogenic function in many more types of cancers. Further, targeting LIF has been actively investigated as a novel strategy for cancer therapy. Extracellular LIF stimulates JAK/STAT, MAPK, and PI3K signaling, forming a 1:1:1 ternary complex. Intracellularly, both chains of the LIF receptor (LIFRβ and gp130) are bound to JAK1, the tyrosine kinase that initiates the signaling cascade. JAK1 phosphorylates five tyrosines on each receptor chain, four of these are docking sites for the transcription factor STAT3 (allowing stimulation of the JAK/STAT signaling pathway), whilst the fifth is a docking site for SHP-2 (which stimulates the MAPK pathway), and SOCS3 (which negatively regulates both the JAK/STAT and MAPK pathways). LIF stimulates a mixture of differentiation, survival, and renewal programs, the balance of which determines the cell's fate. In embryonic stem (ES) cells, signaling is skewed towards survival and self-renewal.

Images & Validation

• Application Notes:
  Application Notes: For research use only. Minimum Sample Size: 50μg/vial

Key Properties

• Source: CHO Cell
• Expression System: Eukaryotic cell
• Biological Origin: Human
• Molecular Weight: 19.7kDa
• Purity: ≥95%
• Endotoxins: ≤10 EU/mg

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze - thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 6 months, -20 to -93°C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized Powder
• Disclaimer: For research use only

Alternative Names

Leukemia Inhibitory Factor, Differentiation-Stimulating Factor, Cholinergic Differentiation Factor, Melanoma-Derived LPL Inhibitor, LIF Interleukin 6 Family Cytokine, Emfilermin, CDF, DIA, HILDA, MLPLI

Quick Database Links

UniProt

P15018

NCBI Gene ID

3976