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| Catalog Number | orb2992826 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Prolyl endopeptidase FAP is produced by our Mammalian expression system and the target gene encoding Leu26-Asp760 is expressed with a human IgG1 Fc tag at the N-terminus. |
| Tag | N-Fc |
| Form/Appearance | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, pH 8.0. |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| UniProt ID | Q12884 |
| MW | Predicted: 110.9 KDa. Observed: 110-140 KDa, reducing conditions |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human FAP (N-Fc) (Cat#orb2992826) at 5μg/ml (100 μl/well) can bind Anti-Human FAP mAb (Cat#orb2993066)*.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human FAP mAb (Cat#orb2993066) is 4.00 ng/ml. (Regularly tested) |
| Expression Region | Leu26-Asp760 |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Alternative names | FAP; FAPA; DPPIV; SIMP; Fapalpha |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.16-10 ng/mL | |
0.057 ng/mL |
Human | |
0.63-40ng/mL | |
0.38 ng/mL |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 87.9 KDa. Observed: 85-115 KDa, reducing conditions |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) | |
Predicted: 86.1 KDa. Observed: 85-100 KDa, reducing conditions |
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