RARα rabbit pAb

• Catalog Number: orb769867
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RAR alpha
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha. AA range:46-95
• MW: 51
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-RARA antibody, anti-NR1B1 antibody, anti-Reti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HeLa cells, using Retinoic Acid Receptor alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using Retinoic Acid Receptor α antibody.

Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night.

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.