RARα rabbit pAb Antibody

SKU: orb769867

Description

Rabbit polyclonal antibody to RAR alpha

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha. AA range:46-95
• Molecular Weight: 51
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RARA antibody, anti-NR1B1 antibody, anti-Retinoic acid receptor alpha antibody, anti-RAR-alpha antibody, anti-Nuclear receptor subfamily 1 group B member 1 antibody

Western blot analysis of lysates from HeLa cells, using Retinoic Acid Receptor alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using Retinoic Acid Receptor α antibody.

Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night.

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.