RARα (phospho Ser77) rabbit pAb

SKU: orb769866

Description

Rabbit polyclonal antibody to RAR alpha (phospho-Ser77)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of Ser77. AA range:46-95
• Molecular Weight: 45
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RARA antibody, anti-NR1B1 antibody, anti-Retinoic acid receptor alpha antibody, anti-RAR-alpha antibody, anti-Nuclear receptor subfamily 1 group B member 1 antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.