Rabbit anti-TAF1 Antibody

SKU: orb1524710

Description

TAF1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1800 and 1850
• Target: TAF1

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; TAF2A; TAFII250; TAFII-250; OF; transcription factor TFIID p250 polypeptide; N-TAF1; transcription initiation factor TFIID subunit 1; XDP; TBP-associated factor 250 kDa; cell cycle gene 1 protein; BA2R; P250; CCGS; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; DYT3; DYT3/TAF1; KAT4; MRXS33; NSCL2; CCG1

Detection of human TAF1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody orb1524710 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody orb1524710 used for IP at 6 µg per reaction.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of lung carcinoma.Antibody: Rabbit anti-TAF1 antibody (orb1524710) used at 1: 5000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human TAF1 by immunohistochemistry.Sample: FFPE section of bladder carcinoma. Antibody: Rabbit anti-TAF1 antibody (orb1524710) used at 1: 1000 (1 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Quick Database Links

Gene Symbol

TAF1

UniProt

P21675