Rabbit anti-STAT2 Antibody

SKU: orb1524696

Description

STAT2 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 725 and 775
• Target: STAT2
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

IMD44; signal transducer and activator of transcription 2; STAT113; signal transducer and activator of transcription 2, 113kDa; ISGF-3; interferon alpha induced transcriptional activator; P113

Detection of human STAT2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-STAT2 antibody orb1524696 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. STAT2 was also immunoprecipitated by rabbit anti-STAT2 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human STAT2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-STAT2 used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

STAT2

UniProt

P52630

RefSeq (Protein):NP_005410.1