Rabbit anti-STAT2 Antibody

• Catalog Number: orb1524694
• Category: Antibodies
• Description: STAT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 801 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: STAT2
• UniProt ID: P52630
• NCBI: NP_005410.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: IMD44; signal transducer and activator of transcri
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human STAT2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-STAT2 antibody orb1524694 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. STAT2 was also immunoprecipitated by rabbit anti-STAT2 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of human STAT2 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-STAT2 used at a dilution of 1: 1, 000 (1µg/ml) or 1: 200 (1µg/ml). Detection: DAB