Rabbit anti-SHANK3 Antibody

SKU: orb1523477

Description

SHANK3 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1475 and 1525
• Target: SHANK3

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

SH3 and multiple ankyrin repeat domains protein 3; DEL22q13.3; SPANK-2; Shank3; shank postsynaptic density protein; PSAP2; PROSAP2; SCZD15; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2

Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523477) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, MCF-7, SR, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523477) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human SHANK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHANK3 antibody (orb1523477) used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

SHANK3

UniProt

Q9BYB0

RefSeq (Protein):NP_277052.1