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Rabbit anti-SASH1 Antibody
Catalog Number: orb1526931
| Catalog Number | orb1526931 |
|---|---|
| Category | Antibodies |
| Description | SASH1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP |
| Reactivity | Human |
| Immunogen | Between 750 and 800 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - Not recommended. Use rabbit anti-SASH1 antibody A302-265A.; IP - 3 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:250 - 1:1,000 |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | SASH1 |
| UniProt ID | O94885 |
| NCBI | NP_056093.3 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | SH3D6A; dJ323M4.1; proline-glutamate repeat-contai Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody orb1526931 used for IP at 3 µg/mg lysate.
Detection of human SASH1 by immunohistochemistry.Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti-SASH1 (Cat. No. orb1526931) used at a dilution of 1: 1, 000 (1µg/ml) and 1: 400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.
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