Rabbit SASH1 Antibody

SKU: orb1526932

Description

SASH1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. Use rabbit anti-SASH1 antibody A302-265A.; IP - 3 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:250 - 1:1,000
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 750 and 800
• Target: SASH1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

dJ323M4.1; proline-glutamate repeat-containing protein; SAM and SH3 domain-containing protein 1; SH3D6A

Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody orb1526932 used for IP at 3 µg/mg lysate.

Detection of human SASH1 by immunohistochemistry.Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti-SASH1 (Cat. No. orb1526932) used at a dilution of 1: 1, 000 (1µg/ml) and 1: 400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

SASH1

UniProt

O94885

RefSeq (Protein):NP_056093.3