Rabbit anti-RFX1 Antibody

SKU: orb1525559

Description

RFX1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 929 and C-terminus
• Target: RFX1

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

MHC class II regulatory factor RFX; transcription factor RFX1; trans-acting regulatory factor 1; RFX; regulatory factor X, 1 (influences HLA class II expression); Regulatory factor X 1; enhancer factor C; EF-C; EFC; MHC class II regulatory factor RFX1

Detection of human RFX1 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, MCF-7, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for IP at 6 µg per reaction. RFX1 was also immunoprecipitated by a previous lot of this antibody (orb1525559) and a second antibody against a different epitope of RFX1.

Detection of human RFX1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525559) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse RFX1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525559) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB

Detection of mouse RFX1 by western blot. Samples: Whole cell lysate (25 µg) from 3T3-L1, CT26, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525559) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Quick Database Links

Gene Symbol

RFX1

UniProt

P22670

RefSeq (Protein):NP_002909.4