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Rabbit anti-PDZ-GEF1 Antibody
Catalog Number: orb1527469
| Catalog Number | orb1527469 |
|---|---|
| Category | Antibodies |
| Description | PDZ-GEF1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human |
| Immunogen | Between 1000 and 1050 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | PDZ-GEF1 |
| UniProt ID | Q9Y4G8 |
| NCBI | NP_055062.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | RAGEF; RA-GEF; RA-GEF-1; Rap guanine nucleotide ex Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human PDZ-GEF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527469 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527469 used for IP at 6 µg per reaction.
Detection of human PDZ-GEF1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Cat. No. orb1527469) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
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