Rabbit anti-MKK7 Antibody

SKU: orb1523367

Description

MKK7 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1 and 50
• Target: MKK7
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

MEK 7; MKK7; PRKMK7; SAPK kinase 4; SAPKK4; MAPKK7; stress-activated protein kinase kinase 4; MAPKK 7; SAPKK-4; JNK kinase 2; MEK; dual specificity mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAPK/ERK kinase 7; c-Jun N-terminal kinase kinase 2

Detection of human and mouse MKK7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MKK7 antibody orb1523367 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human MKK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MKK7 antibody orb1523367 used for IP at 6 µg/mg lysate.

Detection of human MKK7 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MKK7 (Cat. No. orb1523367) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

MKK7

UniProt

O14733

RefSeq (Protein):NP_660186.1