MEK-7 (phospho Thr275) rabbit pAb

SKU: orb769707

Description

Rabbit polyclonal antibody to MEK-7 (phospho-Thr275)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Thr275. AA range:241-290
• Molecular Weight: 43
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MAP2K7 antibody, anti-JNKK2 antibody, anti-MEK7 antibody, anti-MKK7 antibody, anti-PRKMK7 antibody, anti-SKK4 antibody, anti-Dual specificity mitogen-activated protein kinase kinase 7 antibody, anti-MAP kinase kinase 7 antibody, anti-MAPKK 7 antibody, anti-JNK-activating kinase 2 antibody, anti-MAPK/ERK kinase 7 antibody, anti-MEK 7 antibody, anti-Stress-activated p antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Thr275) Antibody.

Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using MAP2K7 (Phospho-Thr275) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).