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Rabbit anti-Met Recombinant Monoclonal Antibody

Catalog Number: orb1806453

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb1806453
CategoryAntibodies
DescriptionRabbit anti-Met Recombinant Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBLR283L
Tested applicationsFC, ICC, IHC, IP, WB
ReactivityHuman
ImmunogenBetween 1340 and C-terminus
Antibody TypePrimary Antibody
Concentration1000 µg/ml
Dilution rangeWB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetMet
UniProt IDP08581
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Alternative namesHGF/SF receptor; tyrosine-protein kinase Met; SF r
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NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by western blot. Samples: Whole cell lysate (10 µg) from GaMG, T-47D, HT-29, OVCAR-8, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MET recombinant monoclonal antibody (orb1806453) used for IP at 6 µl/mg lysate.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-Met Recombinant Monoclonal Antibody

Detection of human MET by immunocytochemistry. Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806453). Secondary: HRP-conjugated goat anti-rabbit IgG.

  • Rabbit anti-Met Recombinant Monoclonal Antibody [orb1806454]

    FC,  ICC,  IHC,  IP,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μl