Rabbit anti-HSF1 Antibody

SKU: orb1525317

Description

HSF1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 479 and C-terminus
• Target: HSF1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

HSTF1; heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1

Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody orb1525317 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. orb1525322 used at a dilution of 1: 5, 000 (0.2µg/ml) (left) , Cat. No. orb1525320 used at a dilution of 1: 5, 000 (0.2µg/ml) (center) and Cat. No. orb1525317 used at a dilution of 1: 1, 000 (0.2µg/ml) (right). Detection: DAB

Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. orb1525322 used at a dilution of 1: 1, 000 (1µg/ml) (left) , Cat. No. orb1525320 used at a dilution of 1: 1, 000 (1µg/ml) (center) and Cat. No. orb1525317 used at a dilution of 1: 200 (1µg/ml) (right). Detection: DAB

Quick Database Links

Gene Symbol

HSF1

UniProt

Q00613

RefSeq (Protein):NP_005517.1