HSF1 (phospho Ser303) rabbit pAb

• Catalog Number: orb768653
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSF1 (phospho-Ser303)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser303. AA range:270-319
• MW: 82
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HSF1 antibody, anti-HSTF1 antibody, anti-Heat
• Note: For research use only

Western Blot analysis of MCF7 + TNF cells using Phospho-HSF1 (S303) Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from MCF7 cells treated with TNF-alpha 20ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is blocked with the phospho peptide.