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Catalog Number | orb1710825 |
---|---|
Category | Antibodies |
Description | GLI1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR256L |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Between 1056 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 100 µg/ml |
Dilution range | WB: 1:1,000, IP: 20 µl/1 mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | GLI1 |
UniProt ID | P08151 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | glioma-associated oncogene homolog 1 (zinc finger Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human GLI1 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, PC-3, RMS-13, MCF-7, and TOV-112D cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710825) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of human GLI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RMS-13 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710825) used for IP at 20 µl/mg lysate.
IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IP, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |