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Rabbit anti-GLI1 Recombinant Monoclonal Antibody

Catalog Number: orb1710825

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb1710825
CategoryAntibodies
DescriptionGLI1 Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBLR256L
Tested applicationsIP, WB
ReactivityHuman
ImmunogenBetween 1056 and C-terminus
Antibody TypePrimary Antibody
Concentration100 µg/ml
Dilution rangeWB: 1:1,000, IP: 20 µl/1 mg lysate
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetGLI1
UniProt IDP08151
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Alternative namesglioma-associated oncogene homolog 1 (zinc finger
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NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-GLI1 Recombinant Monoclonal Antibody

Detection of human GLI1 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, PC-3, RMS-13, MCF-7, and TOV-112D cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710825) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Rabbit anti-GLI1 Recombinant Monoclonal Antibody

Detection of human GLI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RMS-13 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GLI1 recombinant monoclonal antibody (orb1710825) used for IP at 20 µl/mg lysate.