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Catalog Number | orb1530401 |
---|---|
Category | Antibodies |
Description | FUS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 450 and 500 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | FUS |
UniProt ID | P35637 |
NCBI | NP_004951.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | TLS; HNRNPP2; oncogene FUS; translocated in liposa Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human FUS by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, A-549, and U2OS cells. Antibody: Affinity purified rabbit anti-FUS antibody at 0.1 µg/ml.
Detection of mouse FUS by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-FUS antibody at 0.1 µg/ml.
Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Affinity purified rabbit anti-FUS used at 1:5, 000 (0.2µg/ml).
Detection of human FUS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS used at of 1:5, 000 (0.2µg/ml).
Detection of human FUS by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-FUS antibody.