Rabbit anti-eIF4G1/eIF4GI Antibody

SKU: orb1527810

Description

eIF4G1/eIF4GI Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 550 and 600
• Target: eIF4G1/eIF4GI
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

eIF-4-gamma 1; PARK18; P220; eukaryotic translation initiation factor 4 gamma 1; EIF4GI; EIF4-gamma; EIF-4G1; eIF-4G 1; EIF4G; EIF4F; eucaryotic translation initiation factor 4G

Detection of human eIF4G1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-eIF4G1 antibody orb1527810 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.

Detection of human eIF4G1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-eIF4G1 antibody orb1527810 used for IP at 3 µg/mg lysate.

Detection of human eIF4G1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4G1 (Cat. No. orb1527810) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF4G1/eIF4GI

UniProt

Q04637

RefSeq (Protein):NP_886553.2