Rabbit anti-eIF4AIII/EIF4A3 Antibody

SKU: orb1525658

Description

eIF4AIII/EIF4A3 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 125 and 175
• Target: eIF4AIII/EIF4A3
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

NMP 265; RCPS; NUK34; NMP265; MUK34; hNMP 265; Fal1; Eukaryotic translation initiation factor 4A isoform 3; eukaryotic translation initiation factor 4A; eukaryotic initiation factor 4A-III; eIF4A-III; eIF-4A-III; eIF4AIII; DEAD box protein 48; ATP-dependent RNA helicase DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; nuclear matrix protein 265; DDX48; ATP-dependent RNA helicase eIF4A-3; eukaryotic initiation factor 4A-like NUK-34

Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525658 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525658 used for IP at 3 µg/mg lysate.

Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibodies. Detection: DAB

Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry.Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. orb1525658) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF4AIII/EIF4A3

UniProt

P38919

RefSeq (Protein):NP_055555.1