Rabbit eIF4AIII/EIF4A3 Antibody

SKU: orb1525659

Description

eIF4AIII/EIF4A3 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Frog, Gallus, Porcine, Rat, Xenopus, Zebrafish
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 125 and 175
• Target: eIF4AIII/EIF4A3
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; Fal1; hNMP 265; MUK34; NMP 265; NMP265; nuclear matrix protein 265; NUK34; RCPS

Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525659 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody orb1525659 used for IP at 3 µg/mg lysate.

Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibodies. Detection: DAB

Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry.Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 (Cat. No. orb1525659) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

eIF4AIII/EIF4A3

UniProt

P38919

RefSeq (Protein):NP_055555.1