Rabbit anti-eIF3F Antibody

SKU: orb1525613

Description

eIF3F Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 307 and C-terminus
• Target: eIF3F
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

eIF3-p47; EIF3S5; eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); Eukaryotic translation initiation factor 3 subunit 5; deubiquitinating enzyme eIF3f; eIF3-epsilon; eukaryotic translation initiation factor 3 subunit F; eIF-3-epsilon; eIF3 p47; eIF3f

Detection of human and mouse eIF3F by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody orb1525613 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human eIF3F by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF3F (Cat. No. orb1525613) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

eIF3F

UniProt

O00303

RefSeq (Protein):NP_003745.1