EIF3F Antibody

SKU: orb345550

Description

EIF3F antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000
• Reactivity: Human
• Application Notes:
  This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.
• Purity: This affinity-purified antibody is directed against human eIF3f protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with eIF3f protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against eIF3f from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with eIF3f from these sources. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-eIF3F Antibody, eIF-3 epsilon antibody, eIF3 p47 antibody, eIF3-p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody, Eukaryotic translation initiation factor 3 subunit F, Deubiquitinating enzyme eIF3f

Western blot using Biorbyt's affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.

Quick Database Links

UniProt

O00303

RefSeq:6685511